Isolation of phytoconstituents and evaluation of biological potentials of Berberis hispanica from Algeria

Redouane Lemoui,Khellaf Rebbas,Samir Benayache,Feyza Oke-Altuntas,Labib Noman,Fadila Benayache,Ilham Bencherchar,Ibrahim Demirtas,Samira Benyahia

doi:10.3329/bjp.v13i2.36133

Abstract

The aim of this study was to isolate the phytoconstituents and evaluate the biological activities of Berberis hispanica. Three phenolic compounds (tamarixetin, caffeic acid and rutin) were isolated from B. hispanica. The structures of the pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1D-, 2D-NMR, and HPLC-TOF/MS. In addition, antimicrobial, anti-oxidant and anti-proliferative effects of the extracts, some fractions and isolated compounds were evaluated. The extracts of B. hispanica were evaluated against six bacterial strains and exhibited the highest activity against Klebsiella pneumonia (15 mm at 100 mg/mL). Fraction T36 (IC50<5 μg/mL) from the n-butanol extract displayed higher radical scavenging activity than butylated hydroxytoluene. The isolated compounds were evaluated for their antiproliferative effects against human cervical adenocarcinoma (HeLa) cell line by real time cell analyzer assay and tamarixetin exhibited the remarkable effect (IC50<50 μg/mL) on HeLa cells. This study supports the documented medicinal effects of B. hispanica. Video Clip of Methodology: Antiproliferative Assay: 4 min 31 sec Click to watch

Highlights

Plant is considered as nature’s chemical factory for providing the chemical compounds, and as a source of novel therapeutic compounds (Arora et al, 2003)
NMR measurements were performed on a Bruker Avence III spectrometer in dimethyl sulfoxide (DMSO)-d6, CDCl3 or CD3OD (1H-NMR 600 MHz; 13C-NMR 150 MHz)
HPLC-TOF/MS spectrum was recorded in the negative ion mode on an Agilent 6210 spectrometer

Summary

Introduction

Plant is considered as nature’s chemical factory for providing the chemical compounds, and as a source of novel therapeutic compounds (Arora et al, 2003). Several studies have been reported on the antibacterial and anti-oxidant activities of the plant extracts, which may be due to the presence of the natural phenolic compounds (Silva et al, 2010; Prakash et al, 2009). Phenolic compounds play important roles in plants and disease resistance (Servili and Montedoro, 2002; Silva et al, 2006). The genus Berberis (Berberidaceae) includes about 500 species distributed largely in Europe, Northeastern region of the United States, Asia and Africa (Harish, 2012; Mokhber-Dezfuli et al, 2014). Many studies have been reported on the medicinal properties of this genus. It has been employed in cholecystitis, cholelithiasis, jaundice, dysentery, leishmaniasis, malaria, gall stones, hypertension, ischemic heart disease, cardiac arrhythmias and cardiomyopathies (Mokhber-Dezfuli et al, 2014). Different types of chemical constituents were found in the B. jaeschkeana and B. thunbergii species such as alkaloids, glycosides, flavonoids, steroids, saponins reducing sugars and terpenoids (Hussain et al, 2016; Alamzeb et al, 2013)

Objectives

Methods

Results