Abstract
A new liquid chromatography–mass spectrometry with ion-trap detection method for the determination of clenbuterol, ractopamine, clenpenterol, brombuterol, hydroxyclenbuterol, mapenterol and mabuterol in animal liver and urine is described to decrease ion suppression in both matrices. The developed method consists of an aqueous extraction and a two-step clean-up. Final extract was injected in a liquid chromatograph with ion trap mass spectrometer optimized to obtain MS 3 ions which provided more specificity and better signal to noise ratio in the investigated analytes. Those steps have been essential to reach decision limits (CCα) values below the proposed MRPL values for each substance. The CCα were determined and their values were 0.05 μg kg −1 for clenbuterol, 0.2 μg kg −1 for ractopamine and 0.1 μg kg −1 for the other analytes in liver and the same values in μg L −1 for urine samples.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
