Abstract
Background: Inflammation plays an important role in the pathogenesis of various diseases such as cardiovascular diseases, arthritis, obesity and cancer. Thus, in the present study, we evaluated the anti-inflammatory effect of extract from Panax pseudoginseng (PCE) in LPS-stimulated RAW 264.7 macrophages. Materials and methods: The cells were pretreated with PCE (10, 25, 50, and 100 μg/mL) for 1 h and then treated with LPS 1 μg/mL for an additional 12 h. The concentration of nitric oxide (NO) cell culture media was measured according to the Griess method and protein expression was determined using Western blotting. Results: The results showed that PCE treatment decreased LPS-induced NO production in a dose-dependent manner. In addition, PCE inhibited the protein expression of enzymes as well as pro-inflammatory cytokines such as inducible Nitric Oxide Synthase (iNOS), Cyclooxygenase-2 (COX-2), and Interleukin-1β (IL-1β) in the LPS-stimulated RAW 264.7 cells. Especially, 100 μg/mL of PCE exhibited the strongest inhibitory effects on these proteins expression. Conclusion: Overall, these findings suggest that PCE may be a potent therapeutic agent for the prevention and/or treatment of inflammation-associated diseases. Keywords: Anti-inflammation, Panax pseudoginseng, RAW 264.7 macrophages
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