Abstract
After treatment with nitrous acid, a number of mutants of phage f2 have been isolated that grow only on bacterial hosts known to be permissive for other mutations. These latter include T4 amber mutants, λ hd mutants, mutants of the alkaline-phosphatase structural gene, and mutants of T4 rIIThe mutants fall into two groups. One group lyses the nonpermissive host and produces a normal yield of about 10,000 defective particles. The other group is without measurable effect on the nonpermissive host. These results indicate at least two cistrons for the phage f2. Their functions are still to be determined.
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