Abstract
Temperature-sensitive mutants of phage f2 have been isolated and classified into three groups by physiological criteria. One group is blocked at an early stage of growth and synthesizes neither phage RNA nor phage antigen at high temperature. A second group of mutants is blocked in a late function, forms antigenic but defective phage particles. The mutation is related to the maturation of phage particles. Mutants in the other group also affect a late function, do not form antigen, and are assumed to be coat protein mutants. These mutants can be related to a series of amber mutants of f2.
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