Hormones induce the metabolic growth and cytotoxin production of Microcystis aeruginosa under terpinolene stress

Abstract

Several organic compounds released into the aquatic environment have a detrimental impact on humans and other organisms. There is a lack of knowledge about natural hormones and herbicides on non-target organisms, including cyanobacteria. In this study, the response of Microcystis aeruginosa to four phytohormones, indole-3-acetic acid (IAA; 10−5), zeatin (ZT; 10−5), abscisic acid (ABA; 10−7), and brassinolide (BRL; 10−9 mol/L), exposed to terpinolene (TPN; (0.44, 0.88, 1.17, or 1.62 mmol/L) at the cellular and genetic levels were investigated. The results showed that TPN could inhibit the growth and photosynthetic activities and stimulate microcystins (MCs) of M. aeruginosa at various levels through the co-occurrence of oxidative stress, antioxidant defense activities, and an imbalance of the antioxidative system. Hormones played critical roles in the growth promotion and photosynthetic activity by enhancing the antioxidant defense mechanisms and MCs production of M. aeruginosa under TPN stress in both hormone and TPN dose-dependent manner. The growth performance and photosynthetic activities of M. aeruginosa were significant with IAA (p < 0.01) and BSL (p < 0.05) compared to ZT and ABA, as TPN concentrations increased. Hormones stimulated the MCs production significantly BSL (p < 0.05) at various levels and protected the cells against TPN-induced oxidative stress and expression of mcyB and mcyD genes involve in MCs synthesis. Our results indicated that hormone contamination in eutrophic lakes might increase the risk of Microcystis aeruginosa bloom and microcystin production with the TPN association.