Abstract
AbstractThe proportion of delayed implanting mouse blastocysts capable of normal development following transfer to pseudopregnant recipients is influenced by the length of the delay period. Thus, approximately one‐third develop normally when transferred on days 5, 9 or 15 (day 1, day of vaginal plug), while only 3% do so when transferred on day 30 (Weitlauf and Greenwald, '68a). In the present experiments, the amount of glycogen in normal and delayed implanting mouse blastocysts was estimated enzymatically to determine whether it plays a limiting role in embryonic survival.Normal blastocysts and delayed implanting blastocysts (i. e., in mice ovariectomized on day 4) contained a similar amount of glycogen on day 5. However, by day 10 the amount of glycogen in the delayed implanting blastocysts dropped to about half that found on day 5 but showed no further decrease between day 10 and day 30. Therefore, the amount of glycogen in delayed implanting mouse blastocysts does not appear to be the limiting factor in embryonic survival.It was further shown that ovarian hormones influenced the amount of glycogen in the blastocysts. Administration of estrogen to ovariectomized mice caused an increase, while progesterone reduced the levels of glycogen.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.