Abstract
DURING an investigation of differences between established lines of human cells in culture, the alkaline phosphatase content was found to vary from high activity in certain epithelial lines to trace or no detectable activity in other epithelial lines. In a series of six HeLa lines the variation in alkaline phosphatase was as great as among cell lineo derived from other tissues and from different species. Among the six lines of uncloned HeLa cells four showed marked ‘constitutive’ alkaline phosphatase activity and two showed trace or no activity (Table 1).
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