Abstract
Aims: Gerbera is very popular as a cut flower in India. Due to it is multi color its very attractive and it has longer vase life. It is a dwarf, stem-less, herbaceous, perennial plant that is commercially propagated by the cell culture.
 Methodology: The research was conducting an investigation into In vitro method used nutrient culture media under control condition. In-vitro method were observed that the effect of 2,4-D +Kinetin and 2,4-D + BAP with different treatment concentrations on callus induction and shoot multiplication under In vitro condition was carried out using leaf explants.
 Results: The result acquired has shown that minimum time (7.18 days) was noted under the treatment combination 2,4-D @ 2.00 mgl-1+ Kinetin @1.50 mgl-1 whereas maximum time (35.57 days) was found of callus with the treatment combination 2,4-D @2.00 mgl-1+ BAP @2.00 mgl-1under control. After the callus induction, the callus was transferred for the establishment of the shoot on different shoot multiplication media showed that time of shoot initiation 7.18 days. shoot per cent was 76.65 in these days also the number of shoots per explant 11.23 along with this, the development of shoot was completed in 11.74 days when Length of the shoot was viewed on 25 days to shoot the length was 2.17 cm, 35 days after 3.60 and 45 days after 4.56 cm. and after that count of shoot per culture was 12.62 were observed on treatment of CHU Media + BAP @ 2.50 mgl-1+Kinetin @ 1.50 mgl-1.
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