Abstract
We aimed to evaluate the effect of chronic hypergravity in a mouse model of allergic asthma and rhinitis. Forty BALB/c mice were divided as: group A (n = 10, control) sensitized and challenged with saline, group B (n = 10, asthma) challenged by intraperitoneal and intranasal ovalbumin (OVA) to induce allergic asthma and rhinitis, and groups C (n = 10, asthma/rotatory control) and D (n = 10, asthma/hypergravity) exposed to 4 weeks of rotation with normogravity (1G) or hypergravity (5G) during induction of asthma/rhinitis. Group D showed significantly decreased eosinophils, neutrophils, and lymphocytes in their BAL fluid compared with groups B and C (p < 0.05). In real-time polymerase chain reaction using lung homogenate, the expression of IL-1β was significantly upregulated (p < 0.001) and IL-4 and IL-10 significantly downregulated (p < 0.05) in group D. Infiltration of inflammatory cells into lung parenchyma and turbinate, and the thickness of respiratory epithelium was significantly reduced in group D (p < 0.05). The expression of Bcl-2 and heme oxygenase-1 were significantly downregulated, Bax and extracellular dismutase significantly upregulated in Group D. Therefore, chronic hypergravity could have a hormetic effect for allergic asthma and rhinitis via regulation of genes involved in antioxidative and proapoptotic pathways. It is possible that we could use hypergravity machinery for treating allergic respiratory disorders.
Highlights
Space physiology, which deals with physiologic changes in space, is an emerging field of research as the need for space exploration increases
Minois suggested that when D. melanogaster was exposed to 2 weeks of hypergravity (3 to 5G), it lived longer[17]
Group C and group D showed significant decreases of serum total IgE compared with group B
Summary
Space physiology, which deals with physiologic changes in space, is an emerging field of research as the need for space exploration increases. For more successful performance of several missions in space, thorough understanding of the physiologic changes associated with spaceflight is quite mandatory. The immune system is one of the most affected biological systems when exposed to space stimuli. Ling et al suggested that when rat bone marrow mesenchymal stem cells were exposed to hypergravity and 5-azacytidine at the same time, their differentiation into cardiomyocytes was significantly improved[4]. Compared with group A, the number of eosinophils, neutrophils, and lymphocytes in BAL fluid was significantly increased in groups B and C (p < 0.05). After exposure to 4 weeks of hypergravity, group D showed significant decreases of all these inflammatory cells in BAL fluid compared with groups B and C (p < 0.05)
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