Horismenus camobiensis (Hym.: Eulophidae), a new hyperparasitoid of Cotesia invirae (Hym.: Braconidae) in Opsiphanes invirae (Lep.: Nymphalidae) pupae.

C. Hansson,V. A. Costa,B. M. C. Castro,G. Salgado-Neto,J. C. Zanuncio

doi:10.1590/1519-6984.249008

Abstract

Horismenus camobiensis sp. nov. (Hymenoptera: Eulophidae), is described based on morphological, molecular and ecological data; this new species of chalcid wasp acts as hyperparasitoid of Opsiphanis invirae (Hübner, 1818) (Lepidoptera: Nymphalidae) in its parasitoid Cotesia invirae Salgado-Neto and Whitfield, 2019 (Hymenoptera: Braconidae). Diagnoses with morphological and molecular characters and illustrations are provided.

Highlights

Horismenus Walker, 1843, with about 420 species, is one of the largest Eulophidae groups and its species act as primary or secondary parasitoid of insects and arachnids (Noyes, 2018; Morales-Silva et al, 2019), mainly in tropical America (Hansson, 2009)
There are records of Cotesia specimens obtained from Opsiphanes invirae (Hübner, 1818) (Lepidoptera: Nymphalidae) larvae that feed on palm trees in in Brazil, Peru and Venezuela (Yu et al, 2016), amongst them, C. invirae (Salgado-Neto and Whitfield, 2019)
The studied Horismenus specimens were reared from C. invirae, which in turn parasitized O. invirae caterpillars on palm trees (Salgado-Neto and Di Mare, 2010; SalgadoNeto, 2013; Salgado-Neto et al, 2019)

Summary

Introduction

Horismenus Walker, 1843, with about 420 species, is one of the largest Eulophidae groups and its species act as primary or secondary parasitoid of insects and arachnids (Noyes, 2018; Morales-Silva et al, 2019), mainly in tropical America (Hansson, 2009). There are records of Cotesia specimens obtained from Opsiphanes invirae (Hübner, 1818) (Lepidoptera: Nymphalidae) larvae that feed on palm trees in in Brazil, Peru and Venezuela (Yu et al, 2016), amongst them, C. invirae (Salgado-Neto and Whitfield, 2019). Four Eulophidae species have been reported as hyperparasitoids in pupae of Cotesia: Oomyzus sokolowskii (Kurdjumov, 1912), Horismenus opsiphanis The PCR product was purified using polyethylene glycol precipitation (PEG) (Schmitz and Riesner, 2006) and sequenced using the Big Dye 3.1 reagent (Life Technologies®) and 3500 xL automatic sequencer (Life Technologies®)

Material and Methods

Molecular identification

Results