Abstract
Aim:Complexity of the method of isolation, cultivation in vitro and the expensive cost of transplantation process of stem cells, it would require an innovation to homing and differentiation of stem cells and increase folliculogenesis. The stem cells homing was achieved through the provision of food or beverages derived from natural materials like honeybee product. Through honeybee product, there will be homing of stem cells and accompany with the sources from the body itself will take place in regeneration of the ovary.Materials and Methods:Female rats model of degenerative ovary was obtained through food fasting but still have drinking water for 5 days. It caused malnutrition and damage of the ovarian tissue. The administration of 50% honeybee product (T1) was performed for 10 consecutive days, while the positive control group (T0+) was fasted and not given honeybee product and the negative control (T0−) not fasted and without honeybee product. Observations were taken for homing of stem cells, raised of folliculogenesis, differentiation of stem cells, and regeneration of the ovarian tissue using routine H&E staining.Results:Homing of stem cells shown the vascular endothelial growth factor and granulocyte colony-stimulating factor expression; enhancement of folliculogenesis was indicated by an increase of follicle dee Graaf count; enhancement of differentiation of stem cells was indicated by growth differentiation factor-9 expression; and regeneration of ovarian tissue indicated by intact ovarian tissue with growing follicles.Conclusion:Honeybee product can be induced endogenous stem cells in regeneration of ovary failure due to malnutrition.
Highlights
The interest of stem cell therapy today and the few decades tends to be greatly increased [1,2,3]
Homing of stem cells shown the vascular endothelial growth factor and granulocyte colony-stimulating factor expression; enhancement of folliculogenesis was indicated by an increase of follicle dee Graaf count; enhancement of differentiation of stem cells was indicated by growth differentiation factor-9 expression; and regeneration of ovarian tissue indicated by intact ovarian tissue with growing follicles
The results of the study are as follows: The effectiveness of honeybee product was based on: (1) Homing of stem cells based on vascular endothelial growth factor (VEGF) and granulocyte colony-stimulating factor (G-CSF) expressions, (2) follicle dee Graaf count, (3) growth differentiation factor-9 (GDF-9) expressions, and (4) regeneration of ovarian tissue
Summary
The interest of stem cell therapy today and the few decades tends to be greatly increased [1,2,3]. The stem cells have tremendous promise to treat a range of diseases. Stem cell transplantation provides new hope in the treatment of various diseases including infertility due to degenerative conditions of the ovary which could not be cured through usual treatment and operative measures [3,4,5,6,7]. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
