Abstract
The optimization of a mobile phase and temperature for RP-HPLC separation of 3,5-dinitrobenzoyl derivatives of linear alcohol ethoxylates. (LAEs) according to the alkyl chain length is reported. Partial inversion of the elution order of ethoxymers with a change of acetonitrile concentration is employed. A decrease of the temperature allows a further improvement in homologue separation. The addition of sodium perchlorate reduces the retention of higher ethoxymers. This is used for final separation improvement. The optimal conditions found were: stationary phase: LiChrospher 100 RP-18 endcapped, 5 μm; column: LiChroCart 250×4 mm I.D.; mobile phase: acetonitrile-water (98:2, v/v) with 0.0011 mol/l sodium perchlorate; flow-rate: 1.5 ml/min; temperature: 5°C; detection: UV absorption at 233 nm. These conditions were successfully applied to the separation of a mixture of C 12–C 18 LAEs and nonylphenol ethoxylates.
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