Abstract
Radioimmunoassays for human prolactin in various biological fluids have been available since 1971. (1–7) Most of these assays have used heterologous systems with prolactin preparations from various sources, i.e. sheep, pig and primate with their corresponding antiserum. (1–5) Friesen et al. (6) find Sinha et al. (7) are the only authors who have described homologous assays using human prolactin as the reference standard and tracer as well and an anti-human prolactin antiserum. In the present study we have established a homologous radioimmunoassay using human pituitary prolactin. The separation method is based on the double antibody solid phase system. (8) Cross reactivity with human growth hormone (HGH), placental lactogen (HPL), the pituitary protein hormones and prolactins of various species were studied as were values found in normal subjects in basal conditions and after a TRH injection. The results of measuring basal levels and performing dynamic tests under various physiological and pathological circumstances will be reported subsequently.
Published Version
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