Abstract
Movement of markers derived from 3H-labelled choline through the intact membrana granulosa has been quantified using scintillation counting. This passage is most readily explained in terms of junctional transfer and may be detected across in excess of 400 cell diameters. Whereas gonadotropins reduce the movement of choline between cumulus cells and the oocyte, that between somatic cells is unaffected. Both CO 2 and the calcium-transporting ionophore A23187 markedly reduce movement of labelled components and the reported effects of CO 2 and Ca 2+ support the conclusions that the gradients are a result of junctional transfer. In addition, morphological examination of CO 2-treated follicles shows a loss of gap junctions.
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