Efficiency of cloned embryo production using different types of cell donor and electric fusion strengths in goats

Abstract

The type of somatic cell used as a cell donor and the electric field strength (EFS) applied for membrane fusion of the reconstructed oocytes are the two important aspects that need to be standardized for somatic cell nuclear transfer (SCNT). In the present study two somatic cells types, namely fibroblast cell grown from ear tissue biopsies of Barbari female goats and cumulus cells were used as somatic donor cells. For fusion of oocyte reconstructed membranes following somatic cell transfer, a dc current of 3 electrical field strength (EFS), i.e., 1.0–1.5; 2.0–2.5; 3 and above 3, were applied. When cumulus cells were used as a nuclear donor, a maximum fusion rate of (55.4 ± 3.9%) was obtained by applying 2.0–2.5 kV/cm dc current. The fusion rate obtained was significantly ( P < 0.05) higher than all the other EFSs treatments of cumulus, as well as fibroblast cell types. The maximum fusion rate (31.9 ± 2.4%) for the fibroblast cell line was observed when an EFS of 2.0–2.5 kV/cm was applied. It could be concluded that the difference in membrane surface properties between the cumulus and fibroblast cell may contribute to the higher fusion rate obtained in cumulus cells for cloned embryo production.