Abstract
A FISH analysis of chromosome 17 homologs in primates suggests that genomic architecture has a direct role in karyotype evolution and in the genomic instability associated with human disease.
Highlights
Chromosomal rearrangements, such as translocations and inversions, are recurrent phenomena during evolution, and both of them are involved in reproductive isolation and speciation
Evolutionary history of human chromosome 17 Chromosome 17 evolution was studied, initially, by two-color fluorescence in situ hybridization (FISH) of 12 single copy human bacterial artificial chromosome (BAC) clones evenly distributed along the chromosome (Table 1)
The cat clones were used in FISH experiments on metaphase spreads of F. catus and marker order was determined
Summary
Chromosomal rearrangements, such as translocations and inversions, are recurrent phenomena during evolution, and both of them are involved in reproductive isolation and speciation. Bacterial artificial chromosome (BAC) clones in FISH experiments, defining the marker order, can be used in phylogenetically related species to reconstruct the evolutionary history of chromosomal rearrangements in extant primates [8,9]. If sequence data are available, overgo probes (two 24mer oligonucleotides that share eight base pairs of complementary sequence at their 3' ends) or specific sequence tagged sites (STSs) are designed in conserved regions of a reference species to recover orthologous clones by library hybridization [10]. Such orthologous probes facilitate comparison between more distant species and the definition of breakpoints. Molecular analysis of breakpoint regions can provide insight into mechanisms involved in karyotype evolution [11,12]
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