Abstract
The probe materials play a significant role in improving the detection efficiency and sensitivity of lateral-flow immunochromatographic test strip (ICTS). Unlike conventional ICTS assay usually uses single-component, solid gold nanoparticles as labeled probes, in our present study, a bimetallic, hollow Au-Ag nanoparticles (NPs) labeled ICTS was successfully developed for the detection of clenbuterol (CLE). The hollow Au-Ag NPs with different Au/Ag mole ratio and tunable size were synthesized by varying the volume ratio of [HAuCl4]:[Ag NPs] via the galvanic replacement reaction. The surface of hollow Ag-Au NPs was functionalized with 11-mercaptoundecanoic acid (MUA) for further covalently bonded with anti-CLE monoclonal antibody. Overall size of the Au-Ag NPs, size of the holes within individual NPs and also Au/Ag mole ratio have been systematically optimized to amplify both the visual inspection signals and the quantitative data. The sensitivity of optimized hollow Au-Ag NPs probes has been achieved even as low as 2 ppb in a short time (within 15 min), which is superior over the detection performance of conventional test strip using Au NPs. The optimized hollow Au-Ag NPs labeled test strip can be used as an ideal candidate for the rapid screening of CLE in food samples.
Highlights
In the past few decades, numerous efforts were devoted to establish various methods for the detection of CLE residues in biological matrices, including liquid chromatographic mass spectrometry[4,5,6], gas chromatographic mass spectrometry[7,8], high performance liquid chromatographic[9,10] and enzyme-linked immunosorbent assay[11]
Bimetallic hollow Au-Ag NPs are synthesized by galvanic replacement reaction where Ag is replaced by Au since Ag has lower reduction potential[28]
The increasing of Au content lead to the larger size of cavity and some Ag-Au NPs cracked into incomplete NPs with small size, which is in good agreement with Transmission electron microscopy (TEM) observation
Summary
In the past few decades, numerous efforts were devoted to establish various methods for the detection of CLE residues in biological matrices, including liquid chromatographic mass spectrometry[4,5,6], gas chromatographic mass spectrometry[7,8], high performance liquid chromatographic[9,10] and enzyme-linked immunosorbent assay[11] These methods have their own advantages in high sensitivity and selectivity, most of the reports are time-consuming, and require tedious sample pretreatment, expensive equipment and skilled workers, which limit its practical use, especially in on-site detection. The sensitivity of optimized hollow Au-Ag NPs probes for the detection of CLE can be achieved even as low as 2 ppb, which is around 10 fold lower than that of solid Au NP-based test strip
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
