Histopathological impact of Ginger loaded nanoparticle versus ginger extract as a novel therapy of experimentally induced acute ulcerative colitis

Abstract

AbstractBackground: Ulcerative colitis (UC) is a debilitating chronic inflammatory bowel disease that is prevalent in Egypt. Current treatment of UC is expensive and has serious side effects. Aim of the Work: To evaluate the effect of ginger loaded nanoparticles (GDNP) compared to ginger extract (GE) as an alternative treatment of acute UC (AUC) in DSS/ ethanol rat model. Material and Methods: Twenty rats were used. AUC was induced by administration of 2% synthetic dextran sulfate sodium (DSS). Rats were divided into 4 groups: control (GP I), AUC (GP II), AUC received 2.5 gm GE (GP III) and AUC received 2.5 gm superparamagnetic@ silver nanoparticles GDNP (GP VI). Colonic and rectal tissue were assessed histologically using Hematoxylin and eosin (H&E), Periodic acid- Schiff (PAS), Toluidine blue, and electron microscopy (EM). Epithelial proliferation and apoptosis were assessed by anti-KI-67 antibody anti BCL2 antibody. Results: GP III and GP IV showed improvement with GDNP and GE compared to control. Gross damage scores were as follows: GPI 0±0, GPII 2.6±1.1, GPIII 1.4±1.1, and GPIV 0±0 with significant variation (P = 0.000). Cumulative histopathological score of GPI, II, III, and IV were 0±0, 9.2±3.3, 3±1.6, and 1±1 respectively with significant correlation (P= 0.000). GPIII and GPIV displayed strong positive nuclear and cytoplasmic staining for KI-67 (GPIII Mean=50±15/ HPF). (GPIV Mean=66±8/ HPF) and BCL2 (Mean=60±16 and 76±9/ HPF) with significant variation (P= 0.000). Conclusion: GDNP has more potential in treatment of AUC compared to GE. Treatment with GDNPs improved signs, reduced apoptosis and enhanced repair of AUC more significantly