Histopathological characterization of Coffea arabica cultivar IPR 106 resistance to Meloidogyne paranaensis

Luciana Harumi Shigueoka,Inês Cristina De Batista Fonseca,Lucas Yuuki Arita,Orazília França Dorigo,Santino Aleandro Da Silva,Gustavo Hiroshi Sera,Andressa Cristina Zamboni Machado

doi:10.1590/1678-992x-2018-0002

Luciana Harumi Shigueoka, Inês Cristina De Batista Fonseca + Show 5 more

Open Access

https://doi.org/10.1590/1678-992x-2018-0002

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Abstract

ABSTRACT: Meloidogyne species are widely distributed in coffee growing areas in Brazil, where M. paranaensis is responsible to significant losses to the crop. One of the main management strategies is the use of resistant cultivars, such as Apoata rootstock and cultivars IPR 100 and IPR 106. However, the parasitic relationship between coffee and M. paranaensis in cultivars carrying resistance genes has been poorly understood. The objective of this paper was to characterize the resistance response of Coffea arabica cv. IPR 106 to M. paranaensis. For this, penetration and histopathological studies were carried out using root tissues parasitized by the nematode. ‘IPR 106’ and ‘Mundo Novo’ (susceptible cultivar) seedlings were inoculated with 4,000 eggs of M. paranaensis and, 15 days after inoculation (DAI), roots were stained with fuchsine to verify the penetration rates of the nematode. Histopathological studies were conducted at 15, 30, 45 and 60 DAI and nematode reproduction was calculated at 120 DAI. ‘IPR 106’ did not avoid completely nematode penetration at 15 DAI, although the number of nematodes was 50 % lower than in Mundo Novo, disregarding the hypothesis of a pre-infectional mechanism of resistance that could prevent nematode penetration completely. However, giant cells and collapsed nematodes were observed in ‘IPR 106’ at 30 DAI, suggesting that the resistance mechanism to M. paranaensis in ‘IPR 106’ involves a post-infectional response and could be mediated by a hypersensitive reaction.

Highlights

Nematodes of Meloidogyne genera Chitwood, 1949, are widely distributed in coffee growing areas in Brazil, where M. paranaensis, Carneiro et al, 1996, accounts for significant losses to the crop (Campos and Villain, 2005)
Meloidogyne species are widely distributed in coffee growing areas in Brazil, where M. paranaensis is responsible to significant losses to the crop
‘IPR 106’ and ‘Mundo Novo’ seedlings were inoculated with 4,000 eggs of M. paranaensis and, 15 days after inoculation (DAI), roots were stained with fuchsine to verify the penetration rates of the nematode

Summary

Introduction

Nematodes of Meloidogyne genera Chitwood, 1949, are widely distributed in coffee growing areas in Brazil, where M. paranaensis, Carneiro et al, 1996, accounts for significant losses to the crop (Campos and Villain, 2005). In Paraná State, Brazil, M. paranaensis is widespread in coffee crops and have a high economic impact on production (Ferraz, 2008). Meloidogyne spp. are sedentary endoparasites that show complex interactions with their hosts, inducing the establishment of highly specialized giant cells and closely associated to the vascular system of plant root system (Bird, 1961). These cells are responsible for pathogen nutrition during its life cycle (Abad et al, 2009). One of the main management strategies in coffee crops is the use of resistant cultivars, which decreases nematode reproduction rates and reduce nematode populations in the soil, especially when integrated with other strategies, such as crop rotation with non-hosts (Roberts, 2002)

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