Abstract
AimTo assess the histopathological changes of rat palatal mucosa exposed to soft reline materials.MethodsForty-five adult female Wistar rats with controlled living conditions and fed ad libitum were employed. Palatal appliances of heat-polymerized acrylic resin Lucitone 550 were manufactured and worn by forty animals during 14 days. Five animals did not use the appliances (G1) and were used to control the appliance influence. One experimental group (n = 10) used the appliances without any relining material (G2) to control the material effect. Three experimental groups (n = 10) received the following soft reline materials below appliances: Dentusoft (G3), Dentuflex (G4), and Trusoft (G5). Appliances from half of each experimental group(n = 5) was immersed in water bath at 55°C for 10 min before use. Animals were slaughtered and the palates were fixed in 10% buffered formalin. Hematoxylin and eosin stained sections of 5 µm were analyzed by computerized planimetry. Cellular compartment, keratin and total epithelial thickness, and basement membrane length were measured to histopathological description.Analysis of variance and Tukey post-hoc test were used to data examination(α = 0.05).ResultsFor heat-treatment groups, G4 showed less elongated ridges at the basal layer interface (p = 0.037) than G2. When comparing the conditions with and without heat-treatment, only G2 showed a significant decrease in the cellular compartment, keratin layer and total epithelium thickness (p<0.05).ConclusionThe post-polymerization for Lucitone 550 was an effective method to reduce the changes in the rat palatal mucosa. The soft reline materials tested did not cause significant histopathological changes in the rat palatal mucosa.
Highlights
Resilient liners are recommended to decrease traumas caused by the complete denture on the oral mucosa, to fibromucosous tissue conditioning, denture stabilizing and ridge healing after surgery, or as a temporary base material during the osseointegration of dental implants, and several procedures to implant-retained overdentures holding [1,2,3,4,5,6]
There were three anestheticrelated deaths among all female adult rats used in this study
Weights were recorded throughout the experiment, one week after the animals commenced the paste diet and 14 days after the fitting of appliances
Summary
Resilient liners are recommended to decrease traumas caused by the complete denture on the oral mucosa, to fibromucosous tissue conditioning, denture stabilizing and ridge healing after surgery, or as a temporary base material during the osseointegration of dental implants, and several procedures to implant-retained overdentures holding [1,2,3,4,5,6]. Relining materials may be classified based on time of use as temporary and permanent. They may be divided according to their composition in acrylic-based or silicone-based materials [1,6]. The acrylic-based resilient lining materials recommended for relining technique are mostly auto-polymerized materials comprising a liquid mixture of an aromatic ester and ethanol interfused with high polymers [7]. Low or no toxicity related to these compounds has been reported, they possess phthalates, potentially toxic compounds which may promote undesirable biological effects [10] such as reducing male fertility [11], functioning as xenoestrogens [12,13] inducing hormonal tumors, and causing fetal malformations [14,15]
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