Histopathological and Morphometric Study of the Effect of Stanozolol on the Aorta of Male Rats

Abstract

The study aimed to find the histopathological and morphological effects of stanozolol, with different concentrations and periods on the aorta in the male rats. The study comprised 80 white males which set into four groups, with 20 rats for each group. First group set as control group and was left only on tap water and fed with pellets. Second group was injected with stanozolol 25 mg / kgbw and the third group was injected with stanozolol 25 mg / kgbw with vit.E 600 mg / kgbw while fourth group was treated with vit.E 600 mg / kgbw. The period of treatment was extend for 8 weeks, animals were euthanized at fourth, eighth and twelfth week after starting treatment. Histological sections of the aorta in the second group showed the presence of histological changes in the aorta represented by the placement of the fat droplets in all layers of the aortic wall accompanied by the enlarged nuclei of the smooth muscle cells. It was observed that the intensity of histopathological changes increased in week 8 and even at 12 weeks despite of the treatment was interrupted. The proliferation of smooth muscle cells toward the endothelium was observed with endothelial cell enlargement. In the third group, after four and eight weeks, thickening of the aorta lining was found with the deposition of the fat droplets as well as elastic fibers degeneration. The fibers replaced by fat droplets in the middle layer. In the fourth group, no histopathological changes were observed for all periods. The micromorphometric measurements of the all groups presented a significant increment in aortic diameter at a level p≤ 0.05 when compared with the control group.