Detection of some Physiological and Histological changes in laboratory rabbits infected with Necator americanus parasite

Abstract

Background: The present study was conducted for the period from February to October 2022 in University of Samarra laboratories. Methods: This study included 20 male laboratory rabbits, whose weight ranged from (1.4-1.5) Kg, ten of them were as a control group and the other ten were infected with N. americanus parasite. Some biochemical tests such as lipid profile (HDL, Cholesterol, and Triglycerides) and liver enzymes (GOT and GPT) are examened, as well as tissue sections of the liver, lung and small intestine to see the changes that occur due to infection. Results: The present results showed significant differences for total cholesterol between control and infected groups (88.40 and 71.80) mg/dl, respectively, as for triglycerides, non-significant variances were recorded between control and infected groups (68.80 and 69.80) mg/dl, respectively, whereas for HDL the present results showed significant differences between control and infected groups (27.40 and 22.60) mg/dl, respectively. The current results showed significant increase in concentration of GOT of infected group compared with control group (27.20 and 15.60) mg/dl, respectively, as for GPT, non-significant variances were recorded between infected and control groups (17.80 and 14.60) mg/dl, respectively, while for Iron, significant differences occure between infected and control groups (74.00 and 101.40) mg/dl, respectivel. The histological study showed the presence of histological changes in the members infected group that were studied. Conclusion: The occurrence of significant differences in the levels of lipid profils (cholestrerol and HDL) and non- significant differences of triglycerides in infected group compared with control group. The current study recorded significant differences in GOT and Iron, and non- significant differences in GPT of infected group compared with control group. The occurrence of changes in hitological sections of liver, lung and intestine of infected group compared with control group.