Histopathological and enzymatic study on the effect of Aspergillus fumigatus in mice

Abstract

Background: - Aspergillus fumigatus is a ubiquitous and opportunistic fungal pathogen causing sever invasive aspergillosis, an important source of morbidity and mortality inimmunocompromised hosts (human and animals) .The present study was conducted to obtain more clarification about the toxicity and histopathological effects of this pathogen in albino miceMaterial and Methods :- fourteen male mice( 6-10) weeks old ,( 20 – 28) gm weight , divided into two groups , test group ( n= 8) , intranasally infected with 25μl (710 conidia / ml normalsaline ) of A. fumigatus for 7 successive days , compared with uninfected group ( n=6) . the weight of all animals were measured at the beginning and at the end of the experiment , inaddition to histological study for lung , liver and kidney were performed , with estimation of enzyme activity of GOT ( Glutomic Oxaloacetic Transaminase),GPT (Glutamic PyruvicTransaminase) , ALP (Alkaline Phosphatase ) and Creatinine Kinase in homogenized liver and kidney tissues were studied by colorimetric method .Results :- increased ( p< 0.05) in body weight , and organs weight ( lung , liver , kidney ) in infected mice with different histological changes were found in organs tissues of infected miceespecially in lung and liver , these changes were supported by variation in enzyme activities of GOT, GPT, ALP and Creatinin kinase in homogenized organs tissuesConclusion: - normal host can suffer from systematic diseases after inhalation of infective forms of A. Fumigatus (conidia), and further work is needed to study the active components, which are responsible for pathogenesis of this opportunistic fungi