Abstract
IntroductionOsteoarthritis (OA) is a common joint disease that can cause gradual disability among the aging population. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is a key transcription factor that regulates the expression of phase II antioxidant enzymes that provide protection against oxidative stress and tissue damage. The use of histone deacetylase inhibitors (HDACi) has emerged as a potential therapeutic strategy for various diseases. They have displayed chondroprotective effects in various animal models of arthritis. Previous studies have established that Nrf2 acetylation enhances Nrf2 functions. Here we explore the role of Nrf2 in the development of OA and the involvement of Nrf2 acetylation in HDACi protection of OA.MethodsTwo OA models—monosodium iodoacetate (MIA) articular injection and destabilization of the medial meniscus (DMM)—were used with wild-type (WT) and Nrf2-knockout (Nrf2-KO) mice to demonstrate the role of Nrf2 in OA progression. A pan-HDACi, trichostatin A (TSA), was administered to examine the effectiveness of HDACi on protection from cartilage damage. The histological sections were scored. The expression of OA-associated matrix metalloproteinases (MMPs) 1, 3, and 13 and proinflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 were assayed. The effectiveness of HDACi on OA protection was compared between WT and Nrf2-KO mice.ResultsNrf2-KO mice displayed more severe cartilage damage in both the MIA and DMM models. TSA promoted the induction of Nrf2 downstream proteins in SW1353 chondrosarcoma cells and in mouse joint tissues. TSA also reduced the expression of OA-associated proteins MMP1, MMP3, and MMP13 and proinflammatory cytokines TNF-α, IL-1β, and IL-6. TSA markedly reduced the cartilage damage in both OA models but offered no significant protection in Nrf2-KO mice.ConclusionsNrf2 has a major chondroprotective role in progression of OA and is a critical molecule in HDACi-mediated OA protection.
Highlights
Osteoarthritis (OA) is a common joint disease that can cause gradual disability among the aging population
trichostatin A (TSA) reduced the expression of OA-associated proteins MMP1, MMP3, and MMP13 and proinflammatory cytokines tumor necrosis factor alpha (TNF-α), IL-1β, and IL-6
Nuclear factor (erythroid-derived 2)-like 2 (Nrf2)-KO mice display accelerated progression of articular cartilage destruction in monosodium iodoacetate (MIA) and destabilization of the medial meniscus (DMM) models To investigate whether Nrf2 mediates histone deacetylase inhibitors (HDACi) protection against OA, we first tested the role of Nrf2 in the development of OA in the MIA model
Summary
Osteoarthritis (OA) is a common joint disease that can cause gradual disability among the aging population. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is a key transcription factor that regulates the expression of phase II antioxidant enzymes that provide protection against oxidative stress and tissue damage. The use of histone deacetylase inhibitors (HDACi) has emerged as a potential therapeutic strategy for various diseases. They have displayed chondroprotective effects in various animal models of arthritis. Nrf acetylation enhances its transcription capacity and downstream target expression and has been shown to confer protection in animal models of inflammation- and oxidative stress-related disease [7, 8]
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