Histomorphometric Evaluation of a Nanothickness Bioceramic Deposition on Endosseous Implants: A Study in Dogs

Abstract

The objective of this study was to evaluate the bone response to a nanothickness bioceramic ion beam-assisted deposition (IBAD) on endosteal implants in a canine model. Alumina-blasted/acid-etched (control) and IBAD-modified (test) implants were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy + ion beam milling, thin-film mode X-ray diffraction, and atomic force microscope. The implants were surgically placed in four dogs' proximal tibiae and remained for 2 and 4 weeks in vivo. Oxytetracycline (10 mg/kg) was administered for bone labeling 48 hours prior to euthanization. Following euthanization, nondecalcified thin sections were prepared for UV and transmitted light microscopy. The amount of bone labeling was evaluated along the length and away from the implant surface by means of a computer software. The % bone-to-implant contact (BIC) was determined for each specimen. One-way analysis of variance at 95% level of significance along with Tukey's post hoc multiple comparisons were utilized for statistical evaluation. The characterization showed Ca- and P-based amorphous coatings with a 20- to 50-nm thickness. In vivo results showed a significant increase in general and site-specific (to 0.5 mm from the implant surface) bone activity for the 4-week test implants compared with the control implants. Bone activity levels decreased as a function of distance from the implant surface for all groups. No significant differences in BIC were observed between groups. This study showed that both surfaces were biocompatible and osteoconductive and that a time-dependent increase in osteoactivity occurred around the test implants.