Histological culture of Cucurbita maxima and selection of suitable media for induction of callus cultures from seedling members

Abstract

The present study found out the response of leaves, stems , cotyledons and hypocotyls of Cucurbita maxima mg to produce callus on Solid MS medium enriched with different concentrations of auxins such as 2,4-D ,IAA and NAA, and cytokinins such as Kin and BA. The explant of leaves and stems showed high response in the selected medium MSI MS +1.0 mg/L BA +0.5 mg /L NAA) where callus Production from leaves and stems reached 100% in hypocotyls 88% and cotyledons 77% whose calls had the highest fresh weight in this medium 5.2 gm and stems 4.3 gm therefore, the study adopted this medium for its good sustenance of the calls of explants in addition to the high rate of production . The medium MS + 3.0 mg/L Kin + 0.5 mg/L 2,4-D achieved a high production rate for the same explants . However it was excluded since it did not keep the vitality of calls when maintained for along time.