Abstract
To evaluate the protective action of alpha-tocopherol in ischemia/reperfusion injuries of pelvic member of rats. Thirty adult male rats of the Wistar strain were randomized into three experimental groups of 10: Group I--control group with no ischemia or reperfusion. Groups II and III--four hours of ischemia and of hours of reperfusion by means of clamping of the infrarenal aorta. The animals of Group II were treated with saline and those of Group III were treated with i.v. alpha-tocopherol (50 mg/kg). Parameters studied were biopsies of the soleus muscle, dosing of creatine phosphokinase, lactate dehydrogenase, potassium, calcium and arterial blood gasometry. The results of biopsies of the soleus muscles studied by optical microscopy, were not significant in terms of presence of edema among the three groups studied. Variables inflammation and necrosis were not observed, therefore cannot be statistically analyzed. As to dosing of calcium and lactate dehydrogenase, the pH, pO2 and pCO2 values were not significant for all groups studied. We observed that the levels of potassium (Group II > Group I, Fcalculated = 5.84; Fcritical = 3.33), creatine phosphokinase (Group II > Groups I and III, Hcalculated = 13.92; Hcritical = 5.99) and bicarbonate (Groups I and III > Group II, Hcalculated = 11.98; Hcritical = 5.99) presented significant results among groups. From the serum biochemical perspective, the treatment with alpha-tocopherol has attenuated the metabolic injuries in the ischemia/reperfusion syndrome in this experimental model.
Highlights
AnesthesiaIschemia caused by oxygen deficit plays an important role in the pathophysiology of many medical-surgical conditions such as myocardial infarction, hypovolemic shock, bowel obstructions and peripheral vascular disease
Experimental studies have shown that the use of alpha-tocopherol after ischemia/reperfusion in animals attenuated the oxidative injury of the muscle cells and reduced the formation of edema in these cells, which means that they have a partial protective action 8
A significant increase was observed in the levels of potassium (F=5.84) and creatine phosphokinase (CPK) (f=13.92) in Group II compared to Group I and Group III (a-tocopherol) (Tables 1 and 2)
Summary
AnesthesiaIschemia caused by oxygen deficit plays an important role in the pathophysiology of many medical-surgical conditions such as myocardial infarction, hypovolemic shock, bowel obstructions and peripheral vascular disease. Experimental trials have shown the efficacy of drugs to prevent or attenuate ischemia/ reperfusion injuries. Experimental studies have shown that the use of alpha-tocopherol after ischemia/reperfusion in animals attenuated the oxidative injury of the muscle cells and reduced the formation of edema in these cells, which means that they have a partial protective action 8. Considering the importance of acute arterial occlusion and its serious systemic consequences, for instance, the need for occlusion of the infrarenal abdominal aorta in medical-surgical conditions such as the correction of aneurisms, and trauma, an ischemia/reperfusion experimental study was performed in rats subjected to temporary clamping of the infrarenal abdominal aorta, with the purpose of evaluating the following:
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