Histological analysis of the periodontal ligament and alveolar bone during dental movement in diabetic rats subjected to low-level laser therapy

Abstract

ObjectiveThe purpose of this research was to evaluate the histological changes of the periodontal ligament and alveolar bone during dental movement in diabetic rats subjected to low level laser therapy (LLLT). MethodsThe movement of the upper molar was performed in 60male Wistar rats divided into four groups (n=15): CTR (control), DBT (diabetic), CTR/LT (irradiated control) and DBT/LT (irradiated diabetic). Diabetes was induced with alloxan (150mg/kg, i.p.). LLLT was applied with GaAlAs laser at 780nm (35J/cm2). After 7, 13 and 19days, the periodontal ligament and alveolar bone were histologically analyzed. ResultsThe mean of osteoblasts (p<0.01) and blood vessels (p<0.05) were significantly decreased in DBT compared with CTR at 7days, whereas the mean of osteoclasts was lower at 7 (p<0.001) and 13days (p<0.05). In DBT/LT, only the mean of osteoclasts was lower than in CTR (p<0.05) at 7days, but no difference was observed at 13 and 19days (p>0.05). The collagenization of the periodontal ligament was impaired in DBT, whereas DBT/LLT showed density/disposition of the collagen fibers similar to those observed in CTR. ConclusionsLLLT improved the periodontal ligament and alveolar bone remodeling activity in diabetic rats during dental movement.