Abstract

For the research on the neuromuscular disorder, several procedures have widely been employed, i. e. sciatic denervation, sympathetic ganglionectomy, disuse method and some drug administrations. However, the examination of the ischemic skeletal muscle is also considered to be available, though the simple ischemic condition is difficult to produce. It was therefore tried, in this paper, to improve a method for transient ischemia, and to study the morphological and histochemical changes of the skeletal muscles in ischemic state and subsequent recovery period.Histochemical works on the ischemic muscles, previously attempted by Moor, Zimmerman, Scully and Stenger, have been concerned with glycogen, succinic dehydrogenase, alkaline phosphatase and lipid. In this report, however, more kinds of enzymes and other substances were widely investigated.

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