Histochemical Study of the Rat Uterine Glycoconjugate Alteration following Treatment with Exogenous Gonadotropic Hormones during the Implantation Period.

Elham Aliabadi,Fakhreddin Mesbah Ardekani,Zohreh Makoolati,Tahereh Talaei-Khozani,Arvin Aliabadi

doi:10.1155/2020/3967427

Abstract

One of the female causes of infertility is anovulation which is treatable with gonadotropin hormones. These hormones affect the molecular organization of the uterus such as glycoconjugates that are the first site of contact between the blastocyst and the uterus. The objective of this project was to study the alteration of glycoconjugates on the uterine apical, Golgi zone, and basement membrane of epithelial cells and the uterine gland after hyperstimulation with pregnant mare serum gonadotropin (PMSG) (4, 8, 16, 24, and 40 IU), during the implantation period. Injection of PMSG (in experimental groups) and injection of distilled water (in the control group) were followed by HCG administration (10 IU), mating, isolation of positive vaginal plug rats, and killing at 5.5 days of pregnancy. Histochemistry was done on the pregnant uterine horns with the use of WGA, DBA, PNA, ConA, SBA, and UEA lectins. The intensity of the immunohistochemical staining was scored, and quantitative data were generated. 4 IU did not show any significant differences with the control, 8 IU had less effect on the alteration of the Golgi zone, and apical and basement membrane glycoconjugates and 40 IU had the least effects on the alteration of uterine gland glycoconjugates. Also, 24 IU had the most effect on the alteration of uterine glycoconjugates. Understanding of the effects of gonadotropin hormones at the uterine level in implantation time helps to optimize hormonal manipulation for improving the outcome of assisted reproductive procedures. It seems that the optimal dose for superovulation and less alteration in uterine glycoconjugates of rats at implantation time were induced by the administration of 8 IU PMSG.

Highlights

Infertility is a global health issue and affects about 10-15% of reproductive-age couples
4 IU did not show any significant differences with the control, 8 IU had less effect on the alteration of the Golgi zone, and apical and basement membrane glycoconjugates and 40 IU had the least effects on the alteration of uterine gland glycoconjugates
Understanding of the effects of gonadotropin hormones at the uterine level in implantation time helps to optimize hormonal manipulation for improving the outcome of assisted reproductive procedures. It seems that the optimal dose for superovulation and less alteration in uterine glycoconjugates of rats at implantation time were induced by the administration of 8 IU pregnant mare serum gonadotropin (PMSG)

Summary

Introduction

Infertility is a global health issue and affects about 10-15% of reproductive-age couples. One cause of infertility is anovulation which is the most common etiology of female infertility [2, 3] This problem is treatable with the use of gonadotropin hormones in the process of in vitro fertilization (IVF) that result in the formation of supernumerary oocytes [4]. It is known that cellcell interactions are mediated by surface glycoproteins and BioMed Research International the glycocalyx of the surface epithelium [8] Certain carbohydrates such as galactosamine (GalNac), fucose (Fuc), glucosamine (GlcNac), galactose (Gal), and mannose (Man) in the glycocalyx of surface epithelial cells are involved in the attachment and implantation of the blastocyst [9,10,11]. The modulation of carbohydrates that result in the administration of gonadotropin hormones is evaluated with lectins that are proteins of nonimmune origin with a high degree of specificity binding ability to carbohydrates [14]

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