Effect of Pregnant Mare Serum Gonadotropin on Plasma Prolactin, Luteinizing Hormone, Estradiol, and Ovarian Growth in Incubating and Out-of-Lay Turkeys

Abstract

The effect of pregnant mare serum gonadotropin (PMSG) on ovarian growth and estradiol production was assessed in out-of-lay (OL) turkey hens that have low plasma concentrations of prolactin (PRL) and in incubating hens that have high plasma levels of PRL. In OL hens after injection with 400 or 2,000 IU PMSG, plasma concentration of PRL did not change, whereas ovarian weight and plasma concentration of estradiol increased by greater than 20-fold to levels comparable to those of laying hens. Follicles were not arranged in a hierarchy following the injection of either 400 or 2,000 IU PMSG into OL hens.There was a linear relationship between the dose of PMSG injected into incubating hens and the subsequent increase in plasma concentration of estradiol. Plasma levels of PRL were not different among hens injected with 0, 16, 80, or400IU PMSG, whereas plasma levels of PRL decreased in incubating hens injected with 2,000 IU PMSG. A significant increase in ovarian weight occurred only in hens injected with 2,000 IU PMSG. None of the hens deserted the nest following the injection of PMSG, indicating that the maintenance of incubation does not require a steroidogenically quiescent ovary. Although the regressed ovaries of both OL and incubating hens are responsive to gonadotropin stimulation, it would seem that the higher levels of PRL in incubating hens may act, in part, to suppress PMSG-induced ovarian growth and steroidogenesis.