Abstract
In the present investigation, histologic sections of the teeth from control and estrogen-treated rats were stained by various histochemical methods in an effort to determine the influence of estrogenic hormones upon the calcification of dentine. The estrogen-treated dentine stained deep red with periodic acid-Schiff stain and exhibited an abundance of positive alcian blue material which was also metachromatic in nature. These staining reactions would indicate that there was an alteration in the ground substance. This situation produced an interference in the calcification of the dentine.
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