Histochemical Studies on the Hypothalamo-hypophysial Neurosecretory System. III. Vitamin C, Phosphatase, Peroxydase and Zinc

Abstract

Histochemical investigation was made on the hypothalamo-hypophyseal system of dog as to the vitamin C, phosphatase, peroxydase and zinc.1. Nerve cells of neurosecretory nuclei (supraoptic and paraventricular nuclei) were found to contain a large amount of vitamin C.2. Capillary in the hypothalamo-hypophyseal system shows a definitely positive alkaline phosphatase reaction, but this was found to be negative in the neurosecretory cells. In case yeast nucleic acid was used as substrate, some of the pituicytes showed positive reaction.3. Nerve cells of neurosecretory nuclei and some of the pituicytes were found to have markedly great amount of acid phosphase.4. No peroxydase was observed in neurosecretory cells.5. Zinc reaction was found to be highly positive in the pituitary intermediate lobe cells.