Histochemical observations on unique rod-like cells in the developing retina of the normal rat.

Abstract

Our previous immunohistochemical studies using specific anti-opsin antibodies have shown that in the normal postnatal rat retina, opsin-like immunoreactive materials can be seen not only in photoreceptor cells but also in some cells of the inner nuclear layer (INL). To characterize these ectopic opsin-like immunoreactive cells further, we have now studied developing rat retinae at the light and electron microscopic levels using both opsin immunocytochemistry and 5'-nucleotidase (5'-N) cytochemistry. Since photoreceptor cells are specifically stained in both methods, we investigated whether these methods would also stain some of the cells in the INL. Opsin-like immunoreactive cells in the INL always showed a characteristic nuclear structure, which was clearly distinguished from that of other INL cells and resembled rod cell nucleus because of the presence of conspicuous masses of condensed heterochromatin. Moreover, these opsin-like immunoreactive cells in the INL occasionally also displayed a ciliary process. They could only be seen in the INL until about day 25 of postnatal life. In the INL, 5'-N activity was found exclusively in cells showing rod-like nuclear features, and the timing of appearance and disappearance of 5'-N-positive cells in the INL was similar to that of the opsin-like immunoreactive cells. Numerous degenerating cells were observed in the INL at around day 10, some of which showed 5'-N reaction product at the cell surface. These results indicate that during postnatal development of the rat retina, some cells in the INL express several phenotypic properties characteristic of rod cells. It is suggested that these cells might undergo either cell degeneration or phenotypic conversion during the subsequent maturation of the retina.