Abstract
Sensitized spleen cells were incubated with 51Cr‐labelled target fibroblasts. The interaction of lymphoid cells and target cells was stopped by fixation in formalin vapour. Target cell lysis was evaluated by the release of 51Cr and the cultures were stained for acid β‐glucuronidase and carboxylic ester hydrolase. The sensitized lymphoid cells aggregated around cultured donor‐type, fibroblasts and showed a marked staining for β‐glucuronidase activity within a six‐hour incubation period. Spleen cells of unimmunized mice were negative or weakly positive for β‐glucuronidase. Staining for carboxylic ester hydrolase activity was observed only in spleen cells of allo‐immunized mice. The staining was less intense as compared with the staining for β‐glucuronidase. These observations add further support to the suggestion that lysosomal hydrolases might have a bearing on cell‐mediated target cell injury.
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Schedule a callMore From: Acta Pathologica Microbiologica Scandinavica Section C Immunology
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