HindII, HindIII, and HpaI restriction fragment maps of bacteriophage λ DNA

Abstract

The site-specific restriction endonucleases isolated from Hemophilus influenzae strains Rc (HincII) and Rd (HindII+III), and Hemophilus parainfluenzae (HpaI) were used to digest bacteriophage λ DNA into 34, 40, and 15 specific fragments, respectively. The sites cleaved by each of these enzymes were localized on the λ physical map and the fragments resulting from these cleavages were electrophoretically identified on gels by (1) analysis of the digestion profiles of deletion and transducing derivatives of λ; and (2) digesting individual fragments produced by one restriction endonuclease with another restriction endonuclease. This paper presents the HindII, HindIII, and HpaI restriction fragment maps for the entire λ genome, and the data used to derive these maps for the region of the λ genome between the attachment site (at 57.3% λ) and the right vegetative end (100% β). The data for mapping the left arm of λ may be found in the accompanying paper (Robinson and Landy, 1977).