Abstract
.We present three-dimensional (3-D) high-resolution spectral-domain optical coherence microscopy (SD-OCM) by using a supercontinuum (SC) fiber laser source with 300-nm spectral bandwidth (full-width at half-maximum) in the 1700-nm spectral band. By using low-coherence interferometry with SC light and a confocal detection scheme, we realized lateral and axial resolutions of 3.4 and in tissue (), respectively. This is, to the best of our knowledge, the highest 3-D spatial resolution reported among those of Fourier-domain optical coherence imaging techniques in the 1700-nm spectral band. In our SD-OCM, to enhance the imaging depth, a full-range method was implemented, which suppressed the formation of a coherent ghost image and allowed us to set the zero-delay position inside the samples. We demonstrated the 3-D high-resolution imaging capability of 1700-nm SD-OCM through the measurement of an interference signal from a mirror surface and imaging of a single 200-nm polystyrene bead and a pig thyroid gland. Deep tissue imaging at a depth of up to 1.8 mm was also demonstrated. This is the first demonstration of 3-D high-resolution SD-OCM in the 1700-nm spectral band.
Highlights
Optical coherence microscopy (OCM) is an optical coherence imaging technique based on optical coherence tomography (OCT) combined with confocal microscopy using a highnumerical-aperture (NA) objective lens, which allows us to perform three-dimensional (3-D) high-resolution, label-free imaging of biological specimens without any physical or chemical pretreatment, such as staining or sectioning.[1]
To improve the image acquisition speed and signal detection sensitivity of high-resolution 1700-nm OCM, we developed 3-D high-resolution 1700 nm spectral-domain optical coherence microscopy (SD-OCM) by using an SC fiber laser source with a 300-nm spectral bandwidth (FWHM) in the 1700-nm spectral band
This SD-OCM was based on our TDOCM21 and a full-range high-axial-resolution SD-OCT system operating in the 1700-nm spectral band.[16]
Summary
Optical coherence microscopy (OCM) is an optical coherence imaging technique based on optical coherence tomography (OCT) combined with confocal microscopy using a highnumerical-aperture (NA) objective lens, which allows us to perform three-dimensional (3-D) high-resolution, label-free imaging of biological specimens without any physical or chemical pretreatment, such as staining or sectioning.[1]. To improve the image acquisition speed and signal detection sensitivity of high-resolution 1700-nm OCM, we developed 3-D high-resolution 1700 nm spectral-domain optical coherence microscopy (SD-OCM) by using an SC fiber laser source with a 300-nm spectral bandwidth (FWHM) in the 1700-nm spectral band. This SD-OCM was based on our TDOCM21 and a full-range high-axial-resolution SD-OCT system operating in the 1700-nm spectral band.[16] The full-range method was employed to realize both high axial resolution and large imaging depth. We confirmed that the lateral resolution of our SD-OCM system was 3.4 μm
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