Abstract
A simple procedure is described for separating methyl esters of polyunsaturated fatty acids (PUFAs) by argentation chromatography using silver nitrate-impregnated thin-layer chromatography plates. Esters are resolved into discrete classes containing 2, 3, 4, 5 and 6 double bonds and within each of these groupings esters are separated according to chain length. All commonly encountered ( n - 6)PUFAs from C 18 to C 22 are resolved from each other, as are all commonly encountered ( n - 3)PUFAs from C 18 to C 22. The technique is particularly useful for metabolic studies of the chain elongation and further desaturation of PUFAs by separately incubating isolated cells with [1- 14C]18:2( n - 6) and [1- 14C]18:3( n - 3). R F values for individual PUFAs are very reproducible and recoveries of individual [ 14C]PUFAs for radioassay exceed 90%.
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