High-Quality Draft Genome Sequences for Five Non-O157 Shiga Toxin-Producing Escherichia coli Strains Generated with PacBio Sequencing and Optical Maps.

Rebecca L Lindsey,Vladimir Loparev,Dhwani Batra,Lori Rowe,Lisley Garcia-Toledo,Devon Stripling,Nancy Strockbine,Kristen Knipe,Haley Martin,Eija Trees,Phalasy Juieng

doi:10.1128/genomea.00626-16

Rebecca L Lindsey, Vladimir Loparev + Show 9 more

Open Access

https://doi.org/10.1128/genomea.00626-16

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Abstract

Shiga toxin-producing Escherichia coli (STEC) is a foodborne pathogen. We report here the high-quality draft whole-genome sequences of five STEC strains isolated from clinical cases in the United States. This report is for STEC of serotypes O55:H7, O79:H7, O91:H14, O153:H2, and O156:H25.

Highlights

The overall incidence rate of Shiga toxin-producing Escherichia coli (STEC) infection in the United States in 2011 was 1.8 per 100,000 people
Symptoms of STEC infection range from mild diarrhea to severe bloody diarrhea, which in some individuals can progress to hemolytic-uremic syndrome
More than 100 STEC serotypes are known to cause illness in humans, but most infections are caused by seven serotypes [1, 2]

Summary

Introduction

The overall incidence rate of Shiga toxin-producing Escherichia coli (STEC) infection in the United States in 2011 was 1.8 per 100,000 people. Symptoms of STEC infection range from mild diarrhea to severe bloody diarrhea, which in some individuals can progress to hemolytic-uremic syndrome. More than 100 STEC serotypes are known to cause illness in humans, but most infections are caused by seven serotypes [1, 2]. We report here the availability of five high-quality draft whole-genome sequence assemblies generated by PacBio sequencing and verified using the strain optical map.

Results

Conclusion