Abstract
The analysis of antibiotic susceptibility disks by high-pressure liquid chromatography (HPLC) was investigated. Methods are presented for the potency determination of mecillinam, ampicillin, carbenicillin, and cephalothin alone and in various combinations. Good agreement between HPLC and microbiological data is observed for potency determinations with recoveries of greater than 95%. Relative standard deviations of lower than 2% are recorded for each HPLC method. HPLC methods offer improved accuracy and greater precision when compared to the standard microbiological methods of analysis for susceptibility disks.
Highlights
The analysis of antibiotic susceptibility disks by high-pressure liquid chromatography (HPLC) was investigated
Methods are presented for the potency determination of mecillinam, ampicillin, carbenicillin, and cephalotjiin alone and in various combinations
The antibacterial activity of the compound dur- microbiological methods are available for those ing the assay; large experimental un- single-component disks described in the Code of certainties due to difficulties in the preparation Federal Regulations, mecillinam is an experiof standard disks in the microbiological assay mental drug and no official methods of disk often yield results which are imprecise and analysis have been published for its assay either which may only approximate actual values
Summary
The analysis of antibiotic susceptibility disks by high-pressure liquid chromatography (HPLC) was investigated. A large variety of these tions [2] is a horizontal agar diffusion assay, disks has been prepared (Table 1) to determine using disks of known potency This technique the most clinically effective combinations and has the advantage of conveniently confirming levels to be provided to researchers. The antibacterial activity of the compound dur- microbiological methods are available for those ing the assay; large experimental un- single-component disks described in the Code of certainties due to difficulties in the preparation Federal Regulations, mecillinam is an experiof standard disks in the microbiological assay mental drug and no official methods of disk often yield results which are imprecise and analysis have been published for its assay either which may only approximate actual values.
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