Abstract
The chromatographic characteristics of eighteen DNP-amino acids, of dinitrophenol and dinitroaniline were studied on RP-18 and RP-18 plates eluted with aqueous—organic solutions and with mixtures of organic solvents. The optimum conditions for the separation of the highest number of compounds were studied and a two-dimensional chromatogram is reported. On home-made layers of ammonium tungstophosphate, water, aqueous solutions of ammonium nitrate and nitric acid and water—methanol mixtures were used as eluents to separate the pairs of DNP-amino acids which exhibit the same chromatographic behaviour on RP-8 and RP-18 plates.
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