Abstract
A new and sensitive (TLC) assay has been estimated for quantitation of synthetic binary mixtures of antipsychotic drug Amisulpride (AMS) with antimigarine drug Zolmitriptan (ZOL). These medications co-administered with each other for treatment of chronic migraine headache associated with psychosis. The synthetic mixture has been separated on precoated silica TLC plates G 60 F 254 by using mobile phase which consist of mixture of Chloroform: Ethyl acetate: Methanol: Ammonia in ratio ( 30:30:73:3 v/v/v/v ). The mixture has been determined at λ max 231 nm. Retardation factor (RF) values for AMS and ZOL have been 0.6 and 0.43 respectively. Calibration graphs have been linear in range 50-500 ng/spot for all studied drugs. The detection limits have been 6.04 & 10.5 ng/spot and the quantitation limits have been 18.3 & 31.834 ng/spot for AMS and ZOL respectively. The described assay has been utilized for quantitation of synthetic mixture in pharmaceutical tablet &biological fluids.
Published Version
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