Abstract
A novel label-free photoelectrochemical immunosensor was developed for the determination of prostate-specific antigen (PSA) based on fluorine-doped tin oxide (FTO) electrode modified with anatase and rutile titanium dioxide nanoparticles (TiO2(a-r)) as a platform for the electrodeposition of bismuth sulfide (Bi2S3). The photoelectrochemical response of the TiO2(a-r) platform sensitized with Bi2S3 for the ascorbic acid donor molecule (AA) was about 34-fold higher than that observed in the absence of Bi2S3. The Bi2S3/TiO2(a-r)/FTO platform was modified with PSA antibodies to produce the anti-PSA/Bi2S3/TiO2(a-r)/FTO immunosensor, which was incubated in solutions of PSA antigen to inhibit the photocurrent of the immunosensor for AA. The photocurrent of the anti-PSA/Bi2S3/TiO2(a-r)/FTO immunosensor presented a linear relationship with the logarithm of the PSA concentration ranging from 1 pg mL−1 to 100 ng mL−1 with good sensitivity and a limit of detection of 0.870 pg mL−1. The sensor was successfully employed for the determination of PSA in artificial blood plasma samples, with recoveries between 94.6 and 98.6%.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call