Abstract
A sensitive method was developed for the determination of deuterated and non-deuterated 1-methyl-1,2,3,4-tetrahydro-β-carboline by combined capillary gas cheomatography and negative-ion chemical ionization mass spectrometry. 1-Methyl-1,2,3,4-tetrahydro-β-carboline was converted into a trifluoroacetyl derivative after pretreatment with fluorescamine and extraction with ethyl acetate. The derivative was separated by capillary gas chromatography and determined by selected-ion monitoring. In the determination, [3,3,4,4- 2H 4]-1-methyl-1,2,3,4-tetrahydro-β-carboline was used as an internal standard. The method developed in this work was used for the determination of deuterated and non-deuterated 1-methyl-1,2,3,4-tetrahydro-β-carboline in human urine samples collected before and after administration of [3,3- 2H 2]- l-tryptophan.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Chromatography B: Biomedical Sciences and Applications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
