Abstract
AbstractA simple, highly sensitive, and selective differential pulse voltammetry method for the determination of α-lipoic acid (LA) in pharmaceutical preparations was developed and validated. The method is based on a quasi-reversible, diffusion-controlled, one-electron anodic oxidation of LA on a boron-doped diamond electrode (BDDE) in a McIlvaine (citrate-phosphate, C-PB) buffer solution at pH 3.0. For the first time, this environment was used for LA determination. A linear calibration curve was obtained within the concentration range 5.82 × 10−8to 4.00 × 10−4 mol L−1with a correlation coefficient of 0.9999. The limits of detection was estimated to be 1.94 × 10−8 mol L−1, which is one of the lowest values characteristic of voltammetric and chromatographic methods of LA determination. The proposed procedure is sensitive, accurate, and precise. Its utility was demonstrated in the determination of LA in pharmaceuticals without the need for its separation from the matrices. The results were comparable to those obtained by high performance liquid chromatography reference method and were in good accordance with the once declared by manufacturers. Thus, our method can be considered as an alternative to the dominant chromatographic determinations of α-LA in real samples.
Highlights
Reactive oxygen species (ROS) are molecules with at least one unpaired electron
This equilibrium is established by the action of enzymatic antioxidants: superoxide dismutase, catalase and glutathione peroxidase, and low molecular weight antioxidants of a hydrophilic nature, such as ascorbate, glutathione, cysteine, and hydrophobic ones: vitamin D3, carotenoids, coenzyme Q, and tocopherols [2]. α-Lipoic acid (LA) belongs to a group of compounds that have amphiphilic properties
McIlvaine buffer was used for LA determination
Summary
Reactive oxygen species (ROS) are molecules with at least one unpaired electron Its presence causes these compounds to be highly reactive and dangerous for living organisms. The compounds with antioxidant properties play an important role in maintaining the ROS concentration in the equilibrium state This equilibrium is established by the action of enzymatic antioxidants: superoxide dismutase, catalase and glutathione peroxidase, and low molecular weight antioxidants of a hydrophilic nature, such as ascorbate (vitamin C), glutathione, cysteine, and hydrophobic ones: vitamin D3, carotenoids, coenzyme Q, and tocopherols [2]. It can simultaneously protect the lipid membranes of cells, as well as intercellular spaces into which water-soluble components penetrate Both α-LA and its reduced form called dihydrolipoic acid (DHLA) have antioxidant properties [3,4]
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