Abstract
The laser two-photon excited fluorescence (LTPF) has been applied to a capillary electrophoresis (CE) system using a self mode-locked titanium-sapphire laser. The analytes were coumarine 440 (7-amino-4-methyl coumarine) and DCCS (7-diethyl aminocoumarine-3-carboxylic acid succinimidyl ester). The two-photon excited fluorescence emission spectrum of DCCS was identical to the one-photon excited fluorescence emission spectrum. The LTPF quantum efficiency increased through the addition of methanol to the buffer solution, and, as a result, the detection limit decreased. The detection limit of coumarine 440 was 8.0 amol, this value was as low as that obtained by one-photon excited fluorescence.
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