Highly sensitive detection of dopamine using a graphene functionalized plasmonic fiber-optic sensor with aptamer conformational amplification

Abstract

Surface plasmon resonance (SPR) optical fiber sensors can be used as cost-effective and relatively simple-to-implement alternatives to well established bulky prism configurations for high sensitivity in-situ biomedical measurements. The miniaturized size and remote operation ability offer a multitude of opportunities for single-point sensing in hard-to-reach spaces, even possibly in vivo. The biosensor configuration presented here utilizes a nano-scale metal-coated tilted fiber Bragg grating (TFBG) imprinted in a commercial single mode fiber core with no structural modifications. A unique feature of our TFBG sensor is the use of a single layer graphene coating over the gold-coated fiber surface, functionalized with a selective DNA aptamer for highly sensitive detection of target molecules. We have demonstrated the capture of dopamine molecules by the DNA aptamer, resulting in aptamer well-defined conformational changes in response to dopamine surface affinities. This process amplifies the surface refractive index modulation over the fiber surface to enable precise dopamine concentration measurement in real time via monitoring of the surface plasmon resonance signals. The sensor shows a linear response for dopamine concentration in the range from 10−13 M to 10−8 M with a lower limit of detection of 10−13 M. This limiting concentration is lower than the concentration fluctuations of dopamine in the human brain. The sensor works with minimal cross-sensitivities because of the core mode calibration inherent in the TFBG. Integration of the TFBG with a hypodermic needle should allow similar measurements in vivo, presenting an appealing solution for rapid, low power consumption and highly sensitive detection of analytes at low concentrations in medicine as well as in chemical and environmental monitoring.