Highly-porous diatom biosilica stationary phase for thin-layer chromatography

Abstract

This study showed that a nanostructured, highly-porous stationary phase composed of randomly-deposited biosilica frustules isolated from living cells of diatom Pinnularia sp. significantly improved the conventional thin-layer chromatography (TLC) based separation of the triphenylmethane dyes malachite green and fast green relative to silica gel by two mobile phases (9:1:1 v/v 1-butanol:ethanol:water, 5:1:2 v/v 1-butanol:acetic acid:water). Although both stationary phases were composed of amorphous silica rich in silanol groups with particle size of 10–12 μm, diatom biosilica frustules were highly porous, hollow shells with surface structure dominated by 200 nm pore arrays. Diatom biosilica significantly improved the mobility of both malachite green and fast green, enabling the resolution of these analytes. The diatom biosilica layer had a high void fraction of 96% but reduced the flow velocity and permeability constant by a factor of two relative to silica gel. TLC performance was enhanced, as evidenced by ten-fold reduction in theoretical plate height for both analytes using the 1-butanol:acetic acid:water mobile phase, and an increased difference in retention time between malachite green and fast green (ΔRf = 0.26) using the 1-butanol:ethanol:water mobile phase. Analysis of plate height vs. solvent front position by the modified van Deemter equation suggested that dispersive mass transfer was reduced, leading to improved analyte resolution, and that surface of the frustule decreased boundary layer resistance, leading to increased analyte flux. Overall, the basis for improved chromatographic performance is believed to be the unique nano- and microstructure of the diatom biosilica frustule.