Abstract
Bone tissue engineering is considered a promising approach for repairing, regenerating and orthopedic implants. In this regard, polymeric supportive scaffolds reinforced with bioactive nanomaterials provide unique functions help in substitution of impaired bone tissues. In this constant, various layered double hydroxide (LDH) concentrations (ranging from 0.1 wt% to 10 wt%.) within the poly (ε-caprolactone) (PCL) scaffolds were fabricated successfully through electrospinning. The scaffolds were investigated by XRD, SEM, FTIR, and EDX analyses to study the phase identification, morphology, chemical properties, and elemental composition, respectively. The findings demonstrated that the inclusion of LDH to PCL scaffold increased mechanical tensile strength from 1.63 ± 0.4 to 1.920 ± 0.3 Mpa for the pure PCL and PCL + LDH (1 wt%) scaffolds, respectively. A decrease in the average diameter of PCL nanofibers was observed with the addition of LDH. The enrichment of PCL scaffold with LDH promotes in vitro biomineralization when subjected to simulated body fluid (SBF), indicating the bioactive features of the nanocomposite scaffolds. In addition, increased of LDH leads to improvement in protein adsorption capacity compared to the pure one. Furthermore, the cytocompatibility and cytotoxicity of nanocomposite fibers was assessed using human MG-63 osteoblast-like cells. The LDH/PCL scaffold induced mineral deposition and osteogenic differentiation of MG-63 as evident by an increase in ALP activity in comparison with the pure PCL. Overall, our results revealed that electrospun PCL-LDH nanofiber might be an ideal choice for bone tissue engineering.
Published Version
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